rat anti mouse monoclonal f4 80 (Bio-Rad)
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Rat Anti Mouse Monoclonal F4 80, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 5909 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+f4+80/Rat+anti+Mouse+F4%2F80/10__22203_slash_ecm__v033a11-129-8-16
Average 96 stars, based on 5909 article reviews
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Immunostaining:Article Title: Targeting Protease-activated Receptor 4 (PAR4) Protects Against Acute Kidney Injury (AKI) in Ischemia–Reperfusion Injury Article Snippet: .. Immunofluorescence:Article Title: Macrophage RNA polymerase II subunit 5-mediating protein deficiency exacerbates kidney inflammation and fibrosis. Article Snippet: Chronic kidney disease (CKD) represents a global health challenge characterized by progressive renal functional decline and irreversible structural damage [1].. Epidemiological studies show that CKD affects over 10% of the population worldwide, accounting for nearly 1.7 million annual deaths [2, 3].. Kidney fibrosis is a common pathological feature of CKD and featured with aberrant extracellular matrix accumulation and myofibroblasts activation [4, 5]. Article Title: Pyrimidinergic calcium signaling links tubular metabolism to fibrosis in kidney disease. Article Snippet: .. The following primary antibodies were used for immunofluorescence staining: rabbit anti-PDGFRβ (Abcam, ab32570, 1:300 and 1:500), chicken anti-GFP (Aves, GFP-1020, 1:300 and 1:400), mouse antiαSMA-Cy3 (Sigma, C6198, 1:300 and 1:500), rabbit anti-Fibronectin (Sigma, F3648, 1:200); mouse antiCol1a1 (DSHB, F1C3, 1:100), Article Title: Pyrimidinergic calcium signaling links tubular metabolism to fibrosis in kidney disease Article Snippet: .. The following primary antibodies were used for immunofluorescence staining: rabbit anti-PDGFRβ (Abcam, ab32570, 1:300 and 1:500), chicken anti-GFP (Aves, GFP-1020, 1:300 and 1:400), mouse anti-αSMA-Cy3 (Sigma, C6198, 1:300 and 1:500), rabbit anti-Fibronectin (Sigma, F3648, 1:200); mouse anti-Col1a1 (DSHB, F1C3, 1:100), Staining:Article Title: Pyrimidinergic calcium signaling links tubular metabolism to fibrosis in kidney disease. Article Snippet: .. The following primary antibodies were used for immunofluorescence staining: rabbit anti-PDGFRβ (Abcam, ab32570, 1:300 and 1:500), chicken anti-GFP (Aves, GFP-1020, 1:300 and 1:400), mouse antiαSMA-Cy3 (Sigma, C6198, 1:300 and 1:500), rabbit anti-Fibronectin (Sigma, F3648, 1:200); mouse antiCol1a1 (DSHB, F1C3, 1:100), Article Title: Pyrimidinergic calcium signaling links tubular metabolism to fibrosis in kidney disease Article Snippet: .. The following primary antibodies were used for immunofluorescence staining: rabbit anti-PDGFRβ (Abcam, ab32570, 1:300 and 1:500), chicken anti-GFP (Aves, GFP-1020, 1:300 and 1:400), mouse anti-αSMA-Cy3 (Sigma, C6198, 1:300 and 1:500), rabbit anti-Fibronectin (Sigma, F3648, 1:200); mouse anti-Col1a1 (DSHB, F1C3, 1:100), |
![( A and B ) Representative images of <t>histological</t> <t>F4/80</t> staining of gWAT and iWAT tissues from male (A) and female (B) mice at 8 and 18 weeks from CD, HFD, and 3-day DS/ 18-hour CR groups. ( C and D ) quantitative polymerase chain reaction (qPCR) data showing relative gene expression of macrophage markers (F4/80 and CD68), M1 markers [monocyte chemoattractant protein-1 (MCP1) and TNFα], and M2 markers [macrophage galactose N-acetyl-galactosamine specific lectin 2 (MGL2), CD206, and CD209] in gWAT (C) and iWAT (D) of male mice fed with a CD for 18 weeks, an HFD for 18 weeks, or an HFD for 18 weeks followed by 18 hours of 70% CR while remaining on an HFD formulation ( n = 4). ( E ) Plasma TNFα of male mice fed with a CD for 18 weeks, an HFD for 18 weeks, or an HFD for 18 weeks followed by 18 hours of 70% CR while remaining on an HFD formulation. ( F and G ) Relative mRNA expression of Adrb3 in gWAT (F) or iWAT (G) across diet groups. ( H ) Western blots displaying the expression of ADRB3 in gWAT or iWAT across diet groups. Error bars represent mean ± SEM. n number denotes biological replicates. Significance for (C) and (G) was determined using ordinary one-way ANOVA with Tukey test. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8310/pmc13148310/pmc13148310__sciadv.aed0535-f7.jpg)