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rat anti mouse monoclonal f4 80  (Bio-Rad)


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    Structured Review

    Bio-Rad rat anti mouse monoclonal f4 80
    Rat Anti Mouse Monoclonal F4 80, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 5909 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+f4+80/Rat+anti+Mouse+F4%2F80/10__22203_slash_ecm__v033a11-129-8-16
    Average 96 stars, based on 5909 article reviews
    rat anti mouse monoclonal f4 80 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Immunostaining:

    Article Title: Targeting Protease-activated Receptor 4 (PAR4) Protects Against Acute Kidney Injury (AKI) in Ischemia–Reperfusion Injury
    Article Snippet: .. Rat anti-F4/80 (BioRad, MCA497) and Rabbit anti-Ly6g (1A8, Abcam, ab238132) were used for immunostaining. ..

    Immunofluorescence:

    Article Title: Macrophage RNA polymerase II subunit 5-mediating protein deficiency exacerbates kidney inflammation and fibrosis.
    Article Snippet: Chronic kidney disease (CKD) represents a global health challenge characterized by progressive renal functional decline and irreversible structural damage [1].. Epidemiological studies show that CKD affects over 10% of the population worldwide, accounting for nearly 1.7 million annual deaths [2, 3].. Kidney fibrosis is a common pathological feature of CKD and featured with aberrant extracellular matrix accumulation and myofibroblasts activation [4, 5].

    Article Title: Pyrimidinergic calcium signaling links tubular metabolism to fibrosis in kidney disease.
    Article Snippet: .. The following primary antibodies were used for immunofluorescence staining: rabbit anti-PDGFRβ (Abcam, ab32570, 1:300 and 1:500), chicken anti-GFP (Aves, GFP-1020, 1:300 and 1:400), mouse antiαSMA-Cy3 (Sigma, C6198, 1:300 and 1:500), rabbit anti-Fibronectin (Sigma, F3648, 1:200); mouse antiCol1a1 (DSHB, F1C3, 1:100), rat anti-F4/80 (Biorad, MCA497GA, 1:50), rabbit anti-CDA (Thermo Fisher, PA5-76650, 1:100), mouse anti-Aquaporin 1 (Abcam, ab9566, 1:250). .. On the following day, sections were washed with PBS, and incubated at room temperature for 2 hours with the following secondary antibodies: donkey anti-rabbit-AF647 (711-606-152, Jackson ImmunoResearch Europe Ltd, 1:300 and 1:500), donkey anti-chicken-Cy3 (703-165-15, Jackson, 1:300 and 1:500), goat anti-mouse-AF568 (A11004, Thermo Fisher Scientific, 1:500), goat anti-rat-Cy5 (112-607-003, Jackson ImmunoResearch Europe Ltd, 1:500).

    Article Title: Pyrimidinergic calcium signaling links tubular metabolism to fibrosis in kidney disease
    Article Snippet: .. The following primary antibodies were used for immunofluorescence staining: rabbit anti-PDGFRβ (Abcam, ab32570, 1:300 and 1:500), chicken anti-GFP (Aves, GFP-1020, 1:300 and 1:400), mouse anti-αSMA-Cy3 (Sigma, C6198, 1:300 and 1:500), rabbit anti-Fibronectin (Sigma, F3648, 1:200); mouse anti-Col1a1 (DSHB, F1C3, 1:100), rat anti-F4/80 (Bio Rad, MCA497GA, 1:50), rabbit anti-CDA (Thermo Fisher, PA5-76650, 1:100), mouse anti-Aquaporin 1 (Abcam, ab9566, 1:250). .. On the following day, sections were washed with PBS, and incubated at room temperature for 2 h with the following secondary antibodies: donkey anti-rabbit-AF647 (711-606-152, Jackson ImmunoResearch Europe Ltd, 1:300 and 1:500), donkey anti-chicken-Cy3 (703-165-15, Jackson, 1:300 and 1:500), goat anti-mouse-AF568 (A-11004, Thermo Fisher Scientific, 1:500), goat anti-rat-Cy5 (112-607-003, Jackson ImmunoResearch Europe Ltd, 1:500).

    Staining:

    Article Title: Pyrimidinergic calcium signaling links tubular metabolism to fibrosis in kidney disease.
    Article Snippet: .. The following primary antibodies were used for immunofluorescence staining: rabbit anti-PDGFRβ (Abcam, ab32570, 1:300 and 1:500), chicken anti-GFP (Aves, GFP-1020, 1:300 and 1:400), mouse antiαSMA-Cy3 (Sigma, C6198, 1:300 and 1:500), rabbit anti-Fibronectin (Sigma, F3648, 1:200); mouse antiCol1a1 (DSHB, F1C3, 1:100), rat anti-F4/80 (Biorad, MCA497GA, 1:50), rabbit anti-CDA (Thermo Fisher, PA5-76650, 1:100), mouse anti-Aquaporin 1 (Abcam, ab9566, 1:250). .. On the following day, sections were washed with PBS, and incubated at room temperature for 2 hours with the following secondary antibodies: donkey anti-rabbit-AF647 (711-606-152, Jackson ImmunoResearch Europe Ltd, 1:300 and 1:500), donkey anti-chicken-Cy3 (703-165-15, Jackson, 1:300 and 1:500), goat anti-mouse-AF568 (A11004, Thermo Fisher Scientific, 1:500), goat anti-rat-Cy5 (112-607-003, Jackson ImmunoResearch Europe Ltd, 1:500).

    Article Title: Pyrimidinergic calcium signaling links tubular metabolism to fibrosis in kidney disease
    Article Snippet: .. The following primary antibodies were used for immunofluorescence staining: rabbit anti-PDGFRβ (Abcam, ab32570, 1:300 and 1:500), chicken anti-GFP (Aves, GFP-1020, 1:300 and 1:400), mouse anti-αSMA-Cy3 (Sigma, C6198, 1:300 and 1:500), rabbit anti-Fibronectin (Sigma, F3648, 1:200); mouse anti-Col1a1 (DSHB, F1C3, 1:100), rat anti-F4/80 (Bio Rad, MCA497GA, 1:50), rabbit anti-CDA (Thermo Fisher, PA5-76650, 1:100), mouse anti-Aquaporin 1 (Abcam, ab9566, 1:250). .. On the following day, sections were washed with PBS, and incubated at room temperature for 2 h with the following secondary antibodies: donkey anti-rabbit-AF647 (711-606-152, Jackson ImmunoResearch Europe Ltd, 1:300 and 1:500), donkey anti-chicken-Cy3 (703-165-15, Jackson, 1:300 and 1:500), goat anti-mouse-AF568 (A-11004, Thermo Fisher Scientific, 1:500), goat anti-rat-Cy5 (112-607-003, Jackson ImmunoResearch Europe Ltd, 1:500).



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    Bio-Rad rat anti mouse monoclonal f4 80
    Rat Anti Mouse Monoclonal F4 80, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad f4 80
    ( A and B ) Representative images of <t>histological</t> <t>F4/80</t> staining of gWAT and iWAT tissues from male (A) and female (B) mice at 8 and 18 weeks from CD, HFD, and 3-day DS/ 18-hour CR groups. ( C and D ) quantitative polymerase chain reaction (qPCR) data showing relative gene expression of macrophage markers (F4/80 and CD68), M1 markers [monocyte chemoattractant protein-1 (MCP1) and TNFα], and M2 markers [macrophage galactose N-acetyl-galactosamine specific lectin 2 (MGL2), CD206, and CD209] in gWAT (C) and iWAT (D) of male mice fed with a CD for 18 weeks, an HFD for 18 weeks, or an HFD for 18 weeks followed by 18 hours of 70% CR while remaining on an HFD formulation ( n = 4). ( E ) Plasma TNFα of male mice fed with a CD for 18 weeks, an HFD for 18 weeks, or an HFD for 18 weeks followed by 18 hours of 70% CR while remaining on an HFD formulation. ( F and G ) Relative mRNA expression of Adrb3 in gWAT (F) or iWAT (G) across diet groups. ( H ) Western blots displaying the expression of ADRB3 in gWAT or iWAT across diet groups. Error bars represent mean ± SEM. n number denotes biological replicates. Significance for (C) and (G) was determined using ordinary one-way ANOVA with Tukey test. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
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    Bio-Rad anti f4 80
    ( A and B ) Representative images of <t>histological</t> <t>F4/80</t> staining of gWAT and iWAT tissues from male (A) and female (B) mice at 8 and 18 weeks from CD, HFD, and 3-day DS/ 18-hour CR groups. ( C and D ) quantitative polymerase chain reaction (qPCR) data showing relative gene expression of macrophage markers (F4/80 and CD68), M1 markers [monocyte chemoattractant protein-1 (MCP1) and TNFα], and M2 markers [macrophage galactose N-acetyl-galactosamine specific lectin 2 (MGL2), CD206, and CD209] in gWAT (C) and iWAT (D) of male mice fed with a CD for 18 weeks, an HFD for 18 weeks, or an HFD for 18 weeks followed by 18 hours of 70% CR while remaining on an HFD formulation ( n = 4). ( E ) Plasma TNFα of male mice fed with a CD for 18 weeks, an HFD for 18 weeks, or an HFD for 18 weeks followed by 18 hours of 70% CR while remaining on an HFD formulation. ( F and G ) Relative mRNA expression of Adrb3 in gWAT (F) or iWAT (G) across diet groups. ( H ) Western blots displaying the expression of ADRB3 in gWAT or iWAT across diet groups. Error bars represent mean ± SEM. n number denotes biological replicates. Significance for (C) and (G) was determined using ordinary one-way ANOVA with Tukey test. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
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    Bio-Rad 203 f4 80
    ( A and B ) Representative images of <t>histological</t> <t>F4/80</t> staining of gWAT and iWAT tissues from male (A) and female (B) mice at 8 and 18 weeks from CD, HFD, and 3-day DS/ 18-hour CR groups. ( C and D ) quantitative polymerase chain reaction (qPCR) data showing relative gene expression of macrophage markers (F4/80 and CD68), M1 markers [monocyte chemoattractant protein-1 (MCP1) and TNFα], and M2 markers [macrophage galactose N-acetyl-galactosamine specific lectin 2 (MGL2), CD206, and CD209] in gWAT (C) and iWAT (D) of male mice fed with a CD for 18 weeks, an HFD for 18 weeks, or an HFD for 18 weeks followed by 18 hours of 70% CR while remaining on an HFD formulation ( n = 4). ( E ) Plasma TNFα of male mice fed with a CD for 18 weeks, an HFD for 18 weeks, or an HFD for 18 weeks followed by 18 hours of 70% CR while remaining on an HFD formulation. ( F and G ) Relative mRNA expression of Adrb3 in gWAT (F) or iWAT (G) across diet groups. ( H ) Western blots displaying the expression of ADRB3 in gWAT or iWAT across diet groups. Error bars represent mean ± SEM. n number denotes biological replicates. Significance for (C) and (G) was determined using ordinary one-way ANOVA with Tukey test. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
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    Bio-Rad anti f4 80 antibody
    ( A and B ) Representative images of <t>histological</t> <t>F4/80</t> staining of gWAT and iWAT tissues from male (A) and female (B) mice at 8 and 18 weeks from CD, HFD, and 3-day DS/ 18-hour CR groups. ( C and D ) quantitative polymerase chain reaction (qPCR) data showing relative gene expression of macrophage markers (F4/80 and CD68), M1 markers [monocyte chemoattractant protein-1 (MCP1) and TNFα], and M2 markers [macrophage galactose N-acetyl-galactosamine specific lectin 2 (MGL2), CD206, and CD209] in gWAT (C) and iWAT (D) of male mice fed with a CD for 18 weeks, an HFD for 18 weeks, or an HFD for 18 weeks followed by 18 hours of 70% CR while remaining on an HFD formulation ( n = 4). ( E ) Plasma TNFα of male mice fed with a CD for 18 weeks, an HFD for 18 weeks, or an HFD for 18 weeks followed by 18 hours of 70% CR while remaining on an HFD formulation. ( F and G ) Relative mRNA expression of Adrb3 in gWAT (F) or iWAT (G) across diet groups. ( H ) Western blots displaying the expression of ADRB3 in gWAT or iWAT across diet groups. Error bars represent mean ± SEM. n number denotes biological replicates. Significance for (C) and (G) was determined using ordinary one-way ANOVA with Tukey test. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.
    Anti F4 80 Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A and B ) Representative images of histological F4/80 staining of gWAT and iWAT tissues from male (A) and female (B) mice at 8 and 18 weeks from CD, HFD, and 3-day DS/ 18-hour CR groups. ( C and D ) quantitative polymerase chain reaction (qPCR) data showing relative gene expression of macrophage markers (F4/80 and CD68), M1 markers [monocyte chemoattractant protein-1 (MCP1) and TNFα], and M2 markers [macrophage galactose N-acetyl-galactosamine specific lectin 2 (MGL2), CD206, and CD209] in gWAT (C) and iWAT (D) of male mice fed with a CD for 18 weeks, an HFD for 18 weeks, or an HFD for 18 weeks followed by 18 hours of 70% CR while remaining on an HFD formulation ( n = 4). ( E ) Plasma TNFα of male mice fed with a CD for 18 weeks, an HFD for 18 weeks, or an HFD for 18 weeks followed by 18 hours of 70% CR while remaining on an HFD formulation. ( F and G ) Relative mRNA expression of Adrb3 in gWAT (F) or iWAT (G) across diet groups. ( H ) Western blots displaying the expression of ADRB3 in gWAT or iWAT across diet groups. Error bars represent mean ± SEM. n number denotes biological replicates. Significance for (C) and (G) was determined using ordinary one-way ANOVA with Tukey test. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

    Journal: Science Advances

    Article Title: Integrated analysis of insulin resistance reveals metabolic remodeling following diet switch–triggered calorie reduction

    doi: 10.1126/sciadv.aed0535

    Figure Lengend Snippet: ( A and B ) Representative images of histological F4/80 staining of gWAT and iWAT tissues from male (A) and female (B) mice at 8 and 18 weeks from CD, HFD, and 3-day DS/ 18-hour CR groups. ( C and D ) quantitative polymerase chain reaction (qPCR) data showing relative gene expression of macrophage markers (F4/80 and CD68), M1 markers [monocyte chemoattractant protein-1 (MCP1) and TNFα], and M2 markers [macrophage galactose N-acetyl-galactosamine specific lectin 2 (MGL2), CD206, and CD209] in gWAT (C) and iWAT (D) of male mice fed with a CD for 18 weeks, an HFD for 18 weeks, or an HFD for 18 weeks followed by 18 hours of 70% CR while remaining on an HFD formulation ( n = 4). ( E ) Plasma TNFα of male mice fed with a CD for 18 weeks, an HFD for 18 weeks, or an HFD for 18 weeks followed by 18 hours of 70% CR while remaining on an HFD formulation. ( F and G ) Relative mRNA expression of Adrb3 in gWAT (F) or iWAT (G) across diet groups. ( H ) Western blots displaying the expression of ADRB3 in gWAT or iWAT across diet groups. Error bars represent mean ± SEM. n number denotes biological replicates. Significance for (C) and (G) was determined using ordinary one-way ANOVA with Tukey test. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

    Article Snippet: Tissues were then stained either using haemotoxylin (Pioneer Research Chemicals, Colchester, UK, PRC/R/42) and eosin (Pioneer Research Chemicals, Colchester, UK, PRC/66/1) or F4/80 (1:20, Bio-Rad, Hercules, USA, MCA497).

    Techniques: Staining, Real-time Polymerase Chain Reaction, Gene Expression, Formulation, Clinical Proteomics, Expressing, Western Blot